Review

rabbit polyclonal slc12a1 (Proteintech)

Name: rabbit polyclonal slc12a1
Brand: Proteintech
Rating: 93 (36 reviews)

Proteintech is a verified supplier

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Proteintech rabbit polyclonal slc12a1

A. Single cell RNA-sequencing of two organoids samples of day 21 organoids with RSPO added at day 7 or day 14, integrated and plotted in the first two UMAP dimensions grouped as 4 main clusters. B. Expression distribution of key gene markers of distal nephron and ureteric epithelium. C. Re-analysis of the epithelial cluster (cluster 0) plotted in the first two UMAP dimensions, grouped as 4 main clusters. D. Expression of key markers show populations of early distal nephron (GATA3, CDH2, FGF8), Loop of Henle (IRX1, FXYD2, <t>SLC12A1)</t> and other distal nephron (KCNJ1, DEFB1, TMEM52B) markers. E. Kidney cell classification tool DevKidCC identified the lineage identities of cells in our organoid datasets in comparison to that of Mae et al , highlighting a difference in the epithelium present being distal nephron in our datasets while being ureteric epithelial-like in Mae.

Rabbit Polyclonal Slc12a1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal slc12a1/product/Proteintech
Average 93 stars, based on 36 article reviews

rabbit polyclonal slc12a1 - by Bioz Stars, 2026-02

93/100 stars

Images

1) Product Images from "In vitro plasticity between ureteric epithelial and distal nephron identity and maturity is controlled by extracellular signals"

Article Title: In vitro plasticity between ureteric epithelial and distal nephron identity and maturity is controlled by extracellular signals

Journal: bioRxiv

doi: 10.1101/2025.06.03.657609

Figure Legend Snippet: A. Single cell RNA-sequencing of two organoids samples of day 21 organoids with RSPO added at day 7 or day 14, integrated and plotted in the first two UMAP dimensions grouped as 4 main clusters. B. Expression distribution of key gene markers of distal nephron and ureteric epithelium. C. Re-analysis of the epithelial cluster (cluster 0) plotted in the first two UMAP dimensions, grouped as 4 main clusters. D. Expression of key markers show populations of early distal nephron (GATA3, CDH2, FGF8), Loop of Henle (IRX1, FXYD2, SLC12A1) and other distal nephron (KCNJ1, DEFB1, TMEM52B) markers. E. Kidney cell classification tool DevKidCC identified the lineage identities of cells in our organoid datasets in comparison to that of Mae et al , highlighting a difference in the epithelium present being distal nephron in our datasets while being ureteric epithelial-like in Mae.

Techniques Used: RNA Sequencing, Expressing, Comparison

Image Search Results

Journal: bioRxiv

Article Title: In vitro plasticity between ureteric epithelial and distal nephron identity and maturity is controlled by extracellular signals

doi: 10.1101/2025.06.03.657609

Figure Lengend Snippet: A. Single cell RNA-sequencing of two organoids samples of day 21 organoids with RSPO added at day 7 or day 14, integrated and plotted in the first two UMAP dimensions grouped as 4 main clusters. B. Expression distribution of key gene markers of distal nephron and ureteric epithelium. C. Re-analysis of the epithelial cluster (cluster 0) plotted in the first two UMAP dimensions, grouped as 4 main clusters. D. Expression of key markers show populations of early distal nephron (GATA3, CDH2, FGF8), Loop of Henle (IRX1, FXYD2, SLC12A1) and other distal nephron (KCNJ1, DEFB1, TMEM52B) markers. E. Kidney cell classification tool DevKidCC identified the lineage identities of cells in our organoid datasets in comparison to that of Mae et al , highlighting a difference in the epithelium present being distal nephron in our datasets while being ureteric epithelial-like in Mae.

Article Snippet: The following primary antibodies were used for immunofluorescence at a concentration of 1:300: Goat polyclonal anti-GATA3 (Cat#AF2605; R&D Systems), Mouse monoclonal anti-KRT8 (Cat#AB115959; Abcam), Rabbit polyclonal anti-PAX2 (Cat#71-6000; Zymed Laboratories Inc.), Rat polyclonal anti-αPKC (Cat#sc-216; Santa Cruz), Rabbit monoclonal anti-GATA3 (Cat#5852; Cell Signalling Technology), Sheep polyclonal anti-NEPHRIN (Cat#AF4269; R&D Systems), Mouse monoclonal anti-E-CADHERIN (Cat#610181; BD Biosciences), Biotinylated Lotus tetragonolobus lectin (LTL) (Cat#B-1325; Vector Laboratories), Rabbit polyclonal anti-RFP (Cat#PM005; Medical & Biological Laboratories Co.), Goat polyclonal anti-LHX1 (Cat# sc-19341; Santa Cruz Biotechnology, Inc.), Mouse monoclonal anti-MES1/2/3 (Cat# 39795, ActiveMotif), Rabbit polyclonal anti-UMOD (Cat#BT-590; Biomedical Technologies Inc), Rabbit polyclonal SLC12A1 (Cat#18970-1-AP; Proteintech Group), Rabbit polyclonal SLC12A3 (Cat#LS-C662545; Sapphire Bioscience), Chicken polyclonal anti-GFP (Cat#ab13970; Abcam), Rabbit polyclonal anti-SIX2 (Cat#11562-1-AP; Proteintech Group), Rabbit polyclonal anti-UPK3A (Cat#HPA018415; Sigma-Aldrich), Mouse monoclonal anti-actin (ACTA2) (Cat#A2547; Sigma-Aldrich), Rabbit monoclonal anti-RET (Cat#3223, Cell Signalling Technology), Rabbit polyclonal anti-AQP2 (Cat#AB3274; Chemicon).

Techniques: RNA Sequencing, Expressing, Comparison

H+-ATPase B1 subunit is expressed in apical membrane domains along the early portion of the mouse distal nephron. A and B: immunostaining for H+-ATPase B1 subunit (ATP6V1B1) in mouse kidney cross section with the ATP6V1B1H7659 antibody in the cortex (A) and the medulla (B). In the cortex, expression was evident in the intercalated cells (ICs) as well as in early distal tubular segments. In the outer medulla, both thick ascending limb (TAL) segments and ICs in collecting ducts were positive for ATP6V1B1 with the ATP6V1B1H7659 antibody. Toward the inner medullary portion of the kidney, staining was visible only in the ICs of the collecting duct system. Gray stippled lines demarcate the zones of the medulla. C: representative confocal images showing detection of the ATP6V1B1 with the TAL marker SLC12A1 (green) and the ATP6V1B1H7659 antibody (red). Image from the cortical TAL. Staining is visible in ICs as shown by an arrowhead and in TAL as shown by an asterisk. D: confocal images showing localization of ATP6V1B1 with the ATP6V1B1H7659 antibody (red) with the distal convoluted tubule (DCT) marker, SLC12A3 (green). Again, an arrowhead indicates 1 IC, and a DCT tubule is shown by an asterisk. E: immunofluorescent staining with antibodies directed against the H+-ATPase B1 subunit with the ATP6V1B1H7659 antibody (red) with plasma membrane Ca2+-ATPase 4 (PMCA4; green). Staining is present across the cortical TAL, the DCT (DCT1 and DCT2), in addition to the ICs of the collecting system (arrowhead). F: representative confocal images showing detection of ATP6V1B1 with the ATP6V1B1H7659 antibody (red) with AQP2 (green). ICs are indicated by arrowheads, and early distal nephron tubules are marked with an asterisk. G: confocal images showing immunofluorescence labeling of enhanced green fluorescent protein (eGFP; green) with SLC12A3 in transgenic mice expressing eGFP after a 6.5-kb fragment of the ATP6V1B1 promoter. eGFP expression is visible in the collecting system [including both connecting tubule (CNT) cells and ICs, the latter indicated by arrowhead] but absent from DCT defined by SLC12A3 expression as indicated by an asterisk. H: representative confocal images showing detection of eGFP (green) and ATP6V1B1 (red) with the ATP6V1B1H7659 antibody in ATP6V1B1-eGFP mice. ICs are indicated by arrowheads, and early distal tubular segments are indicated by an asterisk. Weak immunoreactivity for ATP6V1B1 is visible in apical domains of CNT cells that express eGFP (indicated by arrowheads).

Journal: American Journal of Physiology - Renal Physiology

Article Title: H + -ATPase B1 subunit localizes to thick ascending limb and distal convoluted tubule of rodent and human kidney

doi: 10.1152/ajprenal.00539.2017

Figure Lengend Snippet: H+-ATPase B1 subunit is expressed in apical membrane domains along the early portion of the mouse distal nephron. A and B: immunostaining for H+-ATPase B1 subunit (ATP6V1B1) in mouse kidney cross section with the ATP6V1B1H7659 antibody in the cortex (A) and the medulla (B). In the cortex, expression was evident in the intercalated cells (ICs) as well as in early distal tubular segments. In the outer medulla, both thick ascending limb (TAL) segments and ICs in collecting ducts were positive for ATP6V1B1 with the ATP6V1B1H7659 antibody. Toward the inner medullary portion of the kidney, staining was visible only in the ICs of the collecting duct system. Gray stippled lines demarcate the zones of the medulla. C: representative confocal images showing detection of the ATP6V1B1 with the TAL marker SLC12A1 (green) and the ATP6V1B1H7659 antibody (red). Image from the cortical TAL. Staining is visible in ICs as shown by an arrowhead and in TAL as shown by an asterisk. D: confocal images showing localization of ATP6V1B1 with the ATP6V1B1H7659 antibody (red) with the distal convoluted tubule (DCT) marker, SLC12A3 (green). Again, an arrowhead indicates 1 IC, and a DCT tubule is shown by an asterisk. E: immunofluorescent staining with antibodies directed against the H+-ATPase B1 subunit with the ATP6V1B1H7659 antibody (red) with plasma membrane Ca2+-ATPase 4 (PMCA4; green). Staining is present across the cortical TAL, the DCT (DCT1 and DCT2), in addition to the ICs of the collecting system (arrowhead). F: representative confocal images showing detection of ATP6V1B1 with the ATP6V1B1H7659 antibody (red) with AQP2 (green). ICs are indicated by arrowheads, and early distal nephron tubules are marked with an asterisk. G: confocal images showing immunofluorescence labeling of enhanced green fluorescent protein (eGFP; green) with SLC12A3 in transgenic mice expressing eGFP after a 6.5-kb fragment of the ATP6V1B1 promoter. eGFP expression is visible in the collecting system [including both connecting tubule (CNT) cells and ICs, the latter indicated by arrowhead] but absent from DCT defined by SLC12A3 expression as indicated by an asterisk. H: representative confocal images showing detection of eGFP (green) and ATP6V1B1 (red) with the ATP6V1B1H7659 antibody in ATP6V1B1-eGFP mice. ICs are indicated by arrowheads, and early distal tubular segments are indicated by an asterisk. Weak immunoreactivity for ATP6V1B1 is visible in apical domains of CNT cells that express eGFP (indicated by arrowheads).

Article Snippet: Furthermore, the following antibodies were used to determine localization of other acid-base transporters and specific markers for individual nephron segments throughout the renal tubule: 1 ) goat polyclonal antibodies against Aquaporin 2 (AQP2; C-17; Santa Cruz Biotechnology); 2 ) rabbit polyclonal antibodies against NKCC2 (SLC12A1, HPA014967; Sigma-Aldrich); 3 ) rabbit polyclonal antibodies against NCC (SLC12A3 HPA028748; Sigma-Aldrich); 4 ) mouse monoclonal antibody against plasma membrane Ca 2+ -ATPase 4 (PMCA4) (JA9, ab2783; Abcam, Cambridge, UK); 5 ) goat polyclonal antibodies against eGFP (Abcam); 6 ) rabbit polyclonal antibodies against ATP6V1E1 (PA5-29899, Thermo Fisher Scientific, Slangerup, Denmark); 7 ) rabbit polyclonal antibodies against ATP6V1B2 (HPA008147; Sigma-Aldrich); 8 ) rabbit polyclonal antibodies against ATP6V1G1 (PA00135A0Rb; Cusabio Technologies, Houston TX); 9 ) mouse monoclonal antibodies directed against the human thiazide-sensitive NaCl cotransporter encoded by the SLC12A3 gene generated as described in detail previously ( 39 ).

Techniques: Immunostaining, Expressing, Staining, Marker, Immunofluorescence, Labeling, Transgenic Assay

H+-ATPase B1 subunit is expressed along the distal nephron of rat kidney. A and B: immunostaining for H+-ATPase B1 subunit (ATP6V1B1) in rat kidney tissue with the ATP6V1B1H7659 antibody in the cortex (A) and the medulla (B). In the cortex, strong immunoreactivity was seen in the intercalated cells (ICs) but also in apical domains in the early distal tubules. Similar to mouse, thick ascending limb (TAL) segments and ICs in collecting ducts were positive for ATP6V1B1 in the outer medulla. C–F: representative confocal images showing detection of the ATP6V1B1 with the ATP6V1B1H7659 antibody (red) and colocalized with SLC12A1 (C, green), SLC12A3 (D, green), plasma membrane Ca2+-ATPase 4 (PMCA4; E, green), and AQP2 (F, green). Staining was similar to mouse, with IC indicated by arrowheads and early distal tubules indicated by asterisks.

Journal: American Journal of Physiology - Renal Physiology

Article Title: H + -ATPase B1 subunit localizes to thick ascending limb and distal convoluted tubule of rodent and human kidney

doi: 10.1152/ajprenal.00539.2017

Figure Lengend Snippet: H+-ATPase B1 subunit is expressed along the distal nephron of rat kidney. A and B: immunostaining for H+-ATPase B1 subunit (ATP6V1B1) in rat kidney tissue with the ATP6V1B1H7659 antibody in the cortex (A) and the medulla (B). In the cortex, strong immunoreactivity was seen in the intercalated cells (ICs) but also in apical domains in the early distal tubules. Similar to mouse, thick ascending limb (TAL) segments and ICs in collecting ducts were positive for ATP6V1B1 in the outer medulla. C–F: representative confocal images showing detection of the ATP6V1B1 with the ATP6V1B1H7659 antibody (red) and colocalized with SLC12A1 (C, green), SLC12A3 (D, green), plasma membrane Ca2+-ATPase 4 (PMCA4; E, green), and AQP2 (F, green). Staining was similar to mouse, with IC indicated by arrowheads and early distal tubules indicated by asterisks.

Techniques: Immunostaining, Staining

H+-ATPase B1 subunit is expressed along the human distal nephron. A and B: immunostaining for ATP6V1B1 in human kidney with the ATP6V1B1S antibody. Abundant expression was evident in the intercalated cells (ICs), but significant strong immunostaining was also detected in distal tubular segments (A). As in mouse, both thick ascending limb (TAL) segments and ICs in collecting ducts were positive for ATP6V1B1 in the outer medulla (B), while only the ICs of the collecting duct system were stained in the inner medullary portion of the kidney. C and D: immunostaining of the H+-ATPase B1 subunit in kidneys with the ATP6V1B1H7659 antibody in cortex (C) and outer medulla (D). Staining pattern similar to that of ATP6V1B1S was found in human kidney, albeit less intense. E: representative confocal images showing detection of ATP6V1B1 with the ATP6V1B1S antibody (green) and the ATP6V1B1H7659 antibody (red). Staining is visible in ICs as indicated by an arrowhead and in early distal tubular structures as indicated by an asterisk. Early distal tubules are stained by both antibodies in the human kidney. F: confocal images showing localization of ATP6V1B1 with the ATP6V1B1S antibody (red) with SLC12A1 (green). Note clear colocalization between the two epitopes to apical domains in the TAL. G: confocal images showing cellular colocalization between H+-ATPase B1 subunit staining from the ATP6V1B1S antibody (red) with SLC12A3 (green). ICs are indicated by an arrowhead, and colocalization of the epitopes to apical domains in the distal convoluted tubule (DCT) is indicated by asterisks. H: confocal images showing colocalization of the AQP2 protein (green) in the collecting system and ATP6V1B1 with the ATP6V1B1S antibody (red). Arrowhead denotes an IC in a collecting duct, which contains AQP2-positive principal cells, and asterisk indicates ATP6V1B1 in tubule from the early distal nephron.

Journal: American Journal of Physiology - Renal Physiology

Article Title: H + -ATPase B1 subunit localizes to thick ascending limb and distal convoluted tubule of rodent and human kidney

doi: 10.1152/ajprenal.00539.2017

Figure Lengend Snippet: H+-ATPase B1 subunit is expressed along the human distal nephron. A and B: immunostaining for ATP6V1B1 in human kidney with the ATP6V1B1S antibody. Abundant expression was evident in the intercalated cells (ICs), but significant strong immunostaining was also detected in distal tubular segments (A). As in mouse, both thick ascending limb (TAL) segments and ICs in collecting ducts were positive for ATP6V1B1 in the outer medulla (B), while only the ICs of the collecting duct system were stained in the inner medullary portion of the kidney. C and D: immunostaining of the H+-ATPase B1 subunit in kidneys with the ATP6V1B1H7659 antibody in cortex (C) and outer medulla (D). Staining pattern similar to that of ATP6V1B1S was found in human kidney, albeit less intense. E: representative confocal images showing detection of ATP6V1B1 with the ATP6V1B1S antibody (green) and the ATP6V1B1H7659 antibody (red). Staining is visible in ICs as indicated by an arrowhead and in early distal tubular structures as indicated by an asterisk. Early distal tubules are stained by both antibodies in the human kidney. F: confocal images showing localization of ATP6V1B1 with the ATP6V1B1S antibody (red) with SLC12A1 (green). Note clear colocalization between the two epitopes to apical domains in the TAL. G: confocal images showing cellular colocalization between H+-ATPase B1 subunit staining from the ATP6V1B1S antibody (red) with SLC12A3 (green). ICs are indicated by an arrowhead, and colocalization of the epitopes to apical domains in the distal convoluted tubule (DCT) is indicated by asterisks. H: confocal images showing colocalization of the AQP2 protein (green) in the collecting system and ATP6V1B1 with the ATP6V1B1S antibody (red). Arrowhead denotes an IC in a collecting duct, which contains AQP2-positive principal cells, and asterisk indicates ATP6V1B1 in tubule from the early distal nephron.

Techniques: Immunostaining, Expressing, Staining

H+-ATPase B2 subunit is expressed in the early distal nephron in mouse and human kidney. A: immunostaining for H+-ATPase B2 subunit (ATP6V1B2) in mouse kidney showed most abundant expression in the cortical portion of the kidney. Note strong staining of the proximal tubules and distal nephron segments. B: less intense staining was observed in the outer and inner medullary portion of the kidney. Gray stippled lines demarcate the zones of the medulla. C: confocal images showing localization of ATP6V1B2 (red) with SLC12A1 (green). D: confocal images showing cellular colocalization between H+-ATPase B2 subunit staining (red) with SLC12A3 (green). Note localization of ATP6V1B2 to apical domains in the thick ascending limb (TAL) and distal convoluted tubule (DCT) as marked by asterisks. E and F: immunostaining for H+-ATPase B2 subunit in human kidney in the cortex (E) and the medulla (F). Staining is visible in proximal tubules and distal nephron segments in the cortical labyrinth and medullary rays and in the medulla.

Journal: American Journal of Physiology - Renal Physiology

Article Title: H + -ATPase B1 subunit localizes to thick ascending limb and distal convoluted tubule of rodent and human kidney

doi: 10.1152/ajprenal.00539.2017

Figure Lengend Snippet: H+-ATPase B2 subunit is expressed in the early distal nephron in mouse and human kidney. A: immunostaining for H+-ATPase B2 subunit (ATP6V1B2) in mouse kidney showed most abundant expression in the cortical portion of the kidney. Note strong staining of the proximal tubules and distal nephron segments. B: less intense staining was observed in the outer and inner medullary portion of the kidney. Gray stippled lines demarcate the zones of the medulla. C: confocal images showing localization of ATP6V1B2 (red) with SLC12A1 (green). D: confocal images showing cellular colocalization between H+-ATPase B2 subunit staining (red) with SLC12A3 (green). Note localization of ATP6V1B2 to apical domains in the thick ascending limb (TAL) and distal convoluted tubule (DCT) as marked by asterisks. E and F: immunostaining for H+-ATPase B2 subunit in human kidney in the cortex (E) and the medulla (F). Staining is visible in proximal tubules and distal nephron segments in the cortical labyrinth and medullary rays and in the medulla.

Techniques: Immunostaining, Expressing, Staining

Distribution of H+-ATPase E1 subunit in mouse and human kidney. A: immunostaining for H+-ATPase E1 subunit (ATP6V1E1) in mouse kidney revealed abundant expression in cortical nephron segments, including the proximal tubules and distal nephron segments. B: staining appeared less intense in the outer and inner medullary portion of the kidney. Intercalated cells (ICs) stained most strongly. Gray stippled lines demarcate the cortex from the medulla. C and D: confocal images showing localization of ATP6V1E1 (red) with SLC12A1 (green, C) and with SLC12A3 (green, D) to apical domains in the thick ascending limb (TAL) and distal convoluted tubule (DCT). Asterisks mark early distal tubules. E and F: antibodies against the H+-ATPase E1 subunit were used to stain human kidney. Akin to the mouse, staining was most abundant in cortical tubule segments, including proximal tubules and distal tubules. IC staining was strong in both the cortex (E) and the medulla (F).

Journal: American Journal of Physiology - Renal Physiology

Article Title: H + -ATPase B1 subunit localizes to thick ascending limb and distal convoluted tubule of rodent and human kidney

doi: 10.1152/ajprenal.00539.2017

Figure Lengend Snippet: Distribution of H+-ATPase E1 subunit in mouse and human kidney. A: immunostaining for H+-ATPase E1 subunit (ATP6V1E1) in mouse kidney revealed abundant expression in cortical nephron segments, including the proximal tubules and distal nephron segments. B: staining appeared less intense in the outer and inner medullary portion of the kidney. Intercalated cells (ICs) stained most strongly. Gray stippled lines demarcate the cortex from the medulla. C and D: confocal images showing localization of ATP6V1E1 (red) with SLC12A1 (green, C) and with SLC12A3 (green, D) to apical domains in the thick ascending limb (TAL) and distal convoluted tubule (DCT). Asterisks mark early distal tubules. E and F: antibodies against the H+-ATPase E1 subunit were used to stain human kidney. Akin to the mouse, staining was most abundant in cortical tubule segments, including proximal tubules and distal tubules. IC staining was strong in both the cortex (E) and the medulla (F).

Techniques: Immunostaining, Expressing, Staining

H+-ATPase G1 subunit is expressed along the mouse and human distal nephron. A and B: immunostaining for H+-ATPase G1 subunit (ATP6V1G1) in mouse kidney showed expression in the cortical and medullary portion of the kidney. Staining was found in both the proximal tubules and distal nephron segments, with pronounced intercalated cell (IC) staining in both cortex and medulla. Gray stippled lines demarcate the zone of the inner medulla. C and D: confocal images showing localization of ATP6V1G1 (red) with SLC12A1 (green, C) or SLC12A3 (green, D). E and F: immunostaining for H+-ATPase G1 subunit in human kidney in the cortex (E) and the medulla (F).

Journal: American Journal of Physiology - Renal Physiology

Article Title: H + -ATPase B1 subunit localizes to thick ascending limb and distal convoluted tubule of rodent and human kidney

doi: 10.1152/ajprenal.00539.2017

Figure Lengend Snippet: H+-ATPase G1 subunit is expressed along the mouse and human distal nephron. A and B: immunostaining for H+-ATPase G1 subunit (ATP6V1G1) in mouse kidney showed expression in the cortical and medullary portion of the kidney. Staining was found in both the proximal tubules and distal nephron segments, with pronounced intercalated cell (IC) staining in both cortex and medulla. Gray stippled lines demarcate the zone of the inner medulla. C and D: confocal images showing localization of ATP6V1G1 (red) with SLC12A1 (green, C) or SLC12A3 (green, D). E and F: immunostaining for H+-ATPase G1 subunit in human kidney in the cortex (E) and the medulla (F).

Techniques: Immunostaining, Expressing, Staining

Review

Structured Review

Images

1) Product Images from "In vitro plasticity between ureteric epithelial and distal nephron identity and maturity is controlled by extracellular signals"

Similar Products

Image Search Results